Accession Number : AD0686274

Title :   THE USE OF AZO DYE-BOUND COLLAGEN TO MEASURE THE REACTION VELOCITIES OF PROTEOLYTIC ENZYMES.

Descriptive Note : Interim rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Moore,Gerald L.

Report Date : 20 FEB 1969

Pagination or Media Count : 12

Abstract : The insoluble protein-dye complex, Azocoll, can be used to measure proteolytic activity as a function of time. By using the aliquoted method discussed in this report, one 5.0 ml reaction mixture can give up to five points on a standard velocity curve. It has been shown that the ratio of solid to liquid in the reaction mixture can be kept constant to give accurate values when pipetting aliquots from a solid-liquid mixture. We have also shown that Azocoll is more sensitive to trypsin than previously reported and that the 'dye bleeding' in control samples can be kept to a manageable level. (Author)

Descriptors :   (*PEPTIDE HYDROLASES, REACTION KINETICS), TRYPSIN, MEASUREMENT, SENSITIVITY, COLLAGEN, DYES, CONCENTRATION(CHEMISTRY)

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE