Accession Number : AD0687219

Title :   TOXIN FORMATION OF Cl. PERFRINGENS TYPES A AND D ON SYNTHETIC MEDIA,

Corporate Author : ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER WASHINGTON D C

Personal Author(s) : Bychenko,B. D. ; Ivanova,L. G.

Report Date : 01 APR 1969

Pagination or Media Count : 12

Abstract : A synthetic medium is proposed, which contains no protein or peptones, for the study of growth and toxin formation of Cl. perfringens. On this medium, lecithinase, O-Hemolysin, and e-toxin were obtained in sufficient titres for in vitro and in vivo analysis. Maximum titres were obtained between the 4th and 6th hours of culture growth. Protein was not detected until the 3rd to 4th hour of growth, reached a maximum by the 5th to 6th hour, and decreased after 24 hours. Vitamin B complex promoted accelerated growth with normal morphology in the strains studied, but did not enhance toxin formation. (Author)

Descriptors :   (*CLOSTRIDIUM PERFRINGENS, *TOXINS AND ANTITOXINS), CULTURE MEDIA, GROWTH(PHYSIOLOGY), NUTRITION, MORPHOLOGY(BIOLOGY), VITAMIN B COMPLEX, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE