Accession Number : AD0688023

Title :   ANALYSE BIOCHIMIQUE DES FACTEURS DETERMINANT LA CROISSANCE DE TUMEURS CANCEREUSES HUMAINES EN CULTURE D'ORGANES IN VITRO (Biochemical Analysis of Factors Determining the Growth of Human Malignant Tumors in Organ Culture in Vitro),

Corporate Author : COLLEGE DE FRANCE PARIS

Personal Author(s) : Croisille,Yvon ; Mason,James ; Wolff,Emilienne ; Wolff,Etienne

Report Date : 1967

Pagination or Media Count : 10

Abstract : The article presents a general view of biochemical analysis of factors determining organotypic growth of human malignant tumours in vitro. Two human malignant tumours of intestinal origin have been maintained for several years, and these tumours proliferate actively in the form of massive organized nodules, comprised solely of cancer cells and devoid of stroma. They conserve their initial properties. These nodules live on chick embryo organs such as the mesonephros and the liver, with which they are associated on the culture medium. Their growth is such that at each 7-day transfer they can be divided into 2 or 3 fragments, thus their number is either maintained or rapidly increased subject to the need. These nodules are used for the study of the extracts or extract fractions which favour their proliferation in vitro. (Author)

Descriptors :   (*CANCER, IN VITRO ANALYSIS), (*CULTURE MEDIA, GROWTH(PHYSIOLOGY)), INTESTINES, HUMANS, AMINO ACIDS, CHROMATOGRAPHIC ANALYSIS

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE