Accession Number : AD0690698

Title :   A PROTON MAGNETIC RESONANCE STUDY OF THE ANOMERIC SPECIES PRODUCED BY d-GLUCOSIDASES,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB

Personal Author(s) : Eveleigh,D. E. ; Perlin,A. S.

Report Date : 07 OCT 1968

Pagination or Media Count : 8

Abstract : The anomeric configuration of sugars released during enzymic hydrolysis of some D-glucosides and D-glucans in deuterium oxide has been determined by proton magnetic resonance spectroscopy. In some instances, the data are more highly definitive than those provided by methods previously used. The overall results indicate that D-glucosidases and polysaccharases of the endo type yield products in which there is retention of the anomeric configuration of the substrate, whereas polysaccharases of the exo type cause an inversion of anomeric configuration. It is suggested that these steroehemical differences may originate in differing affinities of the enzymes for various regions of the substrates. (Author)

Descriptors :   (*GLYCOSIDE HYDROLASES, *NUCLEAR MAGNETIC RESONANCE), POLYSACCHARIDES, HYDROLYSIS, ENZYMES, LABELED SUBSTANCES, DEUTERIUM COMPOUNDS, CARBOHYDRATES, CANADA

Subject Categories : Biochemistry
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE