Accession Number : AD0695635

Title :   AUTOMATED COLUMN CHROMATOGRAPHIC ANALYSIS OF DEACYLATED PHOSPHOLIPIDS,

Corporate Author : ARMY AEROMEDICAL RESEARCH LAB FORT RUCKER ALA

Personal Author(s) : Wetmur,James G. ; Wilson,Charles R.

Report Date : JUN 1969

Pagination or Media Count : 22

Abstract : A procedure is fully described for isolation and deacylation of phospholipids from serum or tissue. Control experiments are described that ensure maximum yield with minimum degradation. A completely automated system is described for column chromatographic resolution and quantitative analysis of the fractions of a complex mixture of deacylated phospholipids. Control experiments are described that ensure maximum efficiency of chromatographic separation of and maximum sensitivity of analysis of the fractions. Elution profiles for human serum and red cells and for rat liver deacylated phospholipids are shown. All of the seven fractions are identified. (Author)

Descriptors :   (*CHROMATOGRAPHIC ANALYSIS, *PHOSPHOLIPIDS), QUANTITATIVE ANALYSIS, FATIGUE(PHYSIOLOGY), ERYTHROCYTES, BLOOD SERUM

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE