Accession Number : AD0697381

Title :   AN IMPROVED HISTOLOGIC TECHNIC FOR STUDYING PRIMATE RETINA.

Descriptive Note : Final rept. Jun 68-May 69,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Coogan,Philip S. ; Morris,Fred

Report Date : SEP 1969

Pagination or Media Count : 14

Abstract : The histologic technic for preparing primate retina described in this report includes intravascular perfusion with a glutaraldehyde-paraformaldehyde mixture followed by osmium postfixation and Epon embedding. The technic routinely yields histologic preparations of good quality which are suitable for both light and electron microscopy. The Paragon stain used for light microscopy gave excellent differentiation of receptor cell parts and made it easy to distinguish normal from degenerating receptor cells. The intravascular perfusion of fixative prevented artifactual rectinal detachment, thus making this technic useful in studying retinal photic injury. (Author)

Descriptors :   (*RETINA, *HISTOLOGY), PRIMATES, ELECTRON MICROSCOPY, BIOLOGICAL STAINS, BURNS(INJURIES), PATHOLOGY

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE