Accession Number : AD0697382

Title :   AN AUTOMATED METHOD FOR QUANTIFYING URINARY PEPTIDES AND FREE AMINO ACIDS.

Descriptive Note : Final rept. 1965-1967,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Ellis,James P. , Jr. ; Prescott,J. M. ; Welch,B. E.

Report Date : SEP 1969

Pagination or Media Count : 22

Abstract : An automated method is described for the simultaneous quantitative analysis of urinary peptides and free amino acids. A novel ninhydrin reagent rendered the method particularly sensitive for the analysis of peptides containing imino acid residues. The method utilizes the hydrolysis and analytic components of a commercial peptide analyzer and the chromatographic and colorimetric recording components of a commercial amino acid analyzer. The method readily facilitates the analysis of one urine sample per day. A brief account is given of the developmental phase of this methodology. Applicability of the method is demonstrated by duplicate analyses of three samples collected from one subject and single analyses of two samples collected from each of three subjects. These data suggest the method yields reasonably reproducible results and that the quantitative excretion patterns of some peptides and amino acids may differ between subjects but are relatively the same for a given subject. (Author)

Descriptors :   (*URINE, *QUANTITATIVE ANALYSIS), (*PEPTIDES, QUANTITATIVE ANALYSIS), (*AMINO ACIDS, QUANTITATIVE ANALYSIS), CHEMICAL ANALYSIS, AUTOMATIC, HYDROLYSIS, CHROMATOGRAPHIC ANALYSIS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE