Accession Number : AD0698390

Title :   CHROMATOGRAPHIC PROPERTIES OF THE HEMOLYSIN FROM THE CNIDARIAN CHIRONEX FLECKERI,

Corporate Author : DEFENCE STANDARDS LABS MARIBYRNONG (AUSTRALIA)

Personal Author(s) : Crone,H. D. ; Keen,T. E. B.

Report Date : 06 APR 1969

Pagination or Media Count : 9

Abstract : Extracts of tentacles from the jellyfish Chironex fleckeri which possessed strong hemolytic properties were examined by ion exchange chromatography on carboxymethyl and diethylaminoethyl cellulose and by exclusion chromatography on Sephadex G-75 and G-200. In some experiments the hemolytic activity was related quantitatively to the lethality of the samples to mice, before and after the separatory procedure used. It was concluded that the hemolytic activity of the extracts was due to a single component, a protein of molecular weight of approximately 70,000. At least one other toxic component was present, which had a molecular weight of approximately 150,000. Methods for purifying the hemolysin are suggested. (Author)

Descriptors :   (*AQUATIC ANIMALS, *TISSUE EXTRACTS), (*POISONS, CHROMATOGRAPHIC ANALYSIS), INVERTEBRATES, ION EXCHANGE, LETHAL DOSAGE, AUSTRALIA

Subject Categories : Biochemistry
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE