Accession Number : AD0699383

Title :   THE EFFECT OF LASER ENERGY ON CELLS IN TISSUE CULTURE.

Descriptive Note : Annual summary rept. no. 2,

Corporate Author : PASADENA FOUNDATION FOR MEDICAL RESEARCH CALIF DEPT OF LASER BIOLOGY

Personal Author(s) : Rounds,Donald E.

Report Date : 03 DEC 1969

Pagination or Media Count : 21

Abstract : Laser power at 2650 A produced cytolysis and inhibition in the migration of leukocytes. It also inhibited the activity of lactic dehydrogenase, and produced a photoproduct of uridylic acid with a reversible absorption change. Injured cells showed a loss of mitotic activity and an increased frequency of nuclear abnormalities. The focused argon microbeam produced visible lesions in nucleoli which showed a reduced uridine incorporation into RNA. Ablation of the nucleolar organizer sites on chromosomes showed a loss of genetic function in organizing a nucleolus. Concentrations of 0.0001 to 0.01 M ADP could mimic the promotion of blood clotting rates, platelet agglutination, and inhibition of cell growth, which were produced by chemicals that are released from ruby laser-traumatized cells. (Author)

Descriptors :   (*TISSUE CULTURE CELLS, *LASERS), (*COHERENT RADIATION, *RADIATION EFFECTS), ENERGY, CHROMOSOMES, GROWTH(PHYSIOLOGY), DOSAGE, IN VITRO ANALYSIS, BLOOD COAGULATION, INHIBITION, MITOSIS, PATHOLOGY, LACTIC ACID, OXIDOREDUCTASES, MORPHOLOGY(BIOLOGY), ADENOSINE PHOSPHATES, PHOTOSENSITIVITY(BIOLOGICAL), LEUKOCYTES, ULTRAVIOLET RADIATION

Subject Categories : Radiobiology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE