Accession Number : AD0700726

Title :   LEUKOCYTE TYPING IN THE RHESUS MONKEY.

Descriptive Note : Final rept. Nov 67-Oct 68,

Corporate Author : MASON RESEARCH INST WORCESTER MASS DEPT OF IMMUNOLOGY

Personal Author(s) : Bogden,Arthur E. ; Gray,J. H. ; Brule,Marie

Report Date : OCT 1969

Pagination or Media Count : 23

Abstract : With the specific aim of developing a practical methodology for producing a spectrum of leukocyte typing reagents, two methods were applied to cytotoxic antisera developed in the rhesus monkey by immunization with allogeneic leukocytes: (a) absorption analysis: a methodical absorption of aliquots of antiserum by all positive reacting cells of a small test panel of leukocyte donors; and (b) selective absorption: absorption of an antiserum with leukocytes pooled from donors selected on the basis of medium reactivity of their leukocytes with the unabsorbed serum. Although the majority of the 12 reagents that were developed were unquestionably polyspecific, the highly selective quality of the reagents permitted an antigenic profiling of the monkey population so that it was possible to gain a general impression of both the number of leukocyte factors shared by any two individuals and the number by which they differed.

Descriptors :   (*LEUKOCYTES, BLOOD GROUPS), (*BLOOD GROUPS, MONKEYS), IMMUNOLOGY, ANTIGENS + ANTIBODIES, COMPATIBILITY, RADIOBIOLOGY, TRANSPLANTATION, CELLS(BIOLOGY), TOXICITY

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE