Accession Number : AD0702744

Title :   RAPID LIQUID CHROMATOGRAPHY OF NUCLEOTIDES. THE SEPARATION OF PICOMOLE AMOUNTS OF 32Pi, AMP, ADP, AND ATP,

Corporate Author : NAVAL AIR DEVELOPMENT CENTER JOHNSVILLE PA AEROSPACE MEDICAL RESEARCH DEPT

Personal Author(s) : Shmukler,Herman W.

Report Date : 30 DEC 1969

Pagination or Media Count : 21

Abstract : By means of high pressure pellicular anion exchange chromatography picomole amounts of 32Pi, AMP, ADP and ATP were separated in 25 minutes. The method uses a linear concentration gradient (between 0.01 M-0.74 M in 30 minutes) formed from a buffer containing 0.25 M KH2PO4 and 2.20 M KC2. The flow-rate was 24 ml per hour and the operating temperature was 70C. (Author)

Descriptors :   (*CHROMATOGRAPHIC ANALYSIS, *NUCLEOTIDES), (*ION EXCHANGE RESINS, CHROMATOGRAPHIC ANALYSIS), (*ADENOSINE PHOSPHATES, CHROMATOGRAPHIC ANALYSIS), PHOSPHATES, BUFFERS, LABELED SUBSTANCES, PHOSPHORUS COMPOUNDS

Subject Categories : Biochemistry
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE