Accession Number : AD0703176

Title :   THE EFFECT OF METHYLENE BLUE ADDITION TO WHOLE BLOOD DURING PROLONGED STORAGE.

Descriptive Note : Interim rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Kocholaty,Walter F. ; Dawson,R. Ben , Jr. ; Ledford,Edith B. ; Gray,John L. ; Billings,Thomas A.

Report Date : 26 NOV 1969

Pagination or Media Count : 13

Abstract : Addition of methylene blue (MB) in concentrations of 0.001 to 0.00001 M to CPD blood resulted in lowered levels of glutathione and glutathione reductase. The levels of adenosinetriphosphate were not affected by the addition of MB. However, the 2,3-diphosphoglycerate levels remained higher than the control values after 21 to 42 days of storage at all MB concentrations used. Hemoglobin function was better maintained by the two lower concentrations of MB during most of the storage period. The high concentration had an adverse or opposite effect. (Author)

Descriptors :   (*BLOOD, STORAGE), (*METHYLENE BLUE, BLOOD), MEMBRANES(BIOLOGY), PRESERVATION, ERYTHROCYTES, OXYGEN, GLUTATHIONE, ADENOSINE PHOSPHATES, PHOSPHOLIPIDS, BIOCHEMISTRY, ENZYMES

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE