Accession Number : AD0704018

Title :   IMPORTANCE OF CULTURE MEDIUM COMPOSITION IN DETERMINING MICROBE CONTAMINATION OF AIR,

Corporate Author : FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO

Personal Author(s) : Ishchenko,G. N. ; Khamrakulova,K.

Report Date : 20 FEB 1970

Pagination or Media Count : 5

Abstract : The purpose of this work is to clarify the importance of medium composition in bacteriological studies of the air. In parallel air studies using the Krotov device and the jar-cup method the following medias were used: simple meat peptone agar, sugar agar, and agar with rabbit blood. Study of the air in one and the same enclosure was carried out ten times at different periods. The cups were kept in a thermostat at 37 degrees for 40-48 hours, after which the colonies were tabulated and the number of microorganisms in 1 cu m of air were calculated. The composition of the culture medium is of great importance. Both in air seedings using the Krotov device and in using the Jar-cup method, the colonies grew least of all on simple agar. On sugar agar with the jar-cup method, the number of colonies increased from 1.5-6 times. The number of bacteria grew even more on blood agar. It can be recommended that bacteriological contamination of the air should be determined by using only medias with blood.

Descriptors :   (*CULTURE MEDIA, BACTERIA), (*BACTERIA, ATMOSPHERES), DETECTION, CONTAMINATION, BLOOD, LABORATORY EQUIPMENT, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE