Accession Number : AD0706557

Title :   MUTATIONS OF APATHOGENICITY AND SMALL PLAQUES IN THE TICK-BORNE ENCEPHALITIS VIRUS INDUCED BY N-NITROSOMETHYL UREA,

Corporate Author : FORT DETRICK FREDERICK MD

Personal Author(s) : Zasukhina,G. D. ; Rapoport,I. A.

Report Date : 11 MAY 1970

Pagination or Media Count : 9

Abstract : N-nitrosomethyl urea induces 100% mutations of apathogenicity in the virus of tick-borne encephalitis. The mutation from normal to small plaques are encountered 25% less frequently, and are not related to apathogenicity mutations. With respect to the degree of specificity, N-nitrosomethyl urea is superior to 1.4-bis-diasoacethyl butane, since the former evokes two types of mutation whereas 1.4-bis-diasoacethyl butane is characterized by three mutations. The nearly 100% mutation frequency brought about N-nitrosomethyl urea is achieved when the viability of the virus is decreased tenfold, and a 75% mutation frequency is obtained when the viability is decreased fivefold; this has never been observed up to now in experiments with other physical and chemical mutagens. (Author)

Descriptors :   (*NITROSO COMPOUNDS, *MUTATIONS), (*EQUINE ENCEPHALOMYELITIS VIRUS, MUTATIONS), ARBOVIRUSES, UREA, BUTANES, GENETICS, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE