Accession Number : AD0707028

Title :   THE INFLUENCE OF CARBON AND NITROGEN NUTRITION ON GROWTH AND STEROL-INDUCED SEXUALITY OF PYTHIUM SP. PRL 2142,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB

Personal Author(s) : Child,J. J. ; Defago,G. ; Haskins,R. H.

Report Date : 06 AUG 1969

Pagination or Media Count : 10

Abstract : The influence of the major constituents of a chemically defined nutritional medium on growth and sexuality of Pythium sp. PRL 2142 has been determined. Addition of cholesterol to the culture medium stimulated vegetative growth and the response to sterol was quantitative up to a maximum concentration of 60 mg of sterol per liter. The number of oospores produced was proportional to the concentration of sterol within limits determined by the carbon and nitrogen source. Glucose, fructose, maltose and cellobiose were suitable carbon sources for both growth and oospore production whilst alanine, arginine and asparagine proved suitable nitrogen sources. A carbon/nitrogen ratio in the range 15 to 30:1 was optimal for both growth and the production of oospores. Oospore production with asparagine as nitrogen source was at least three times greater than when alanine or arginine was supplied. (Author)

Descriptors :   (*FUNGI, REPRODUCTION(PHYSIOLOGY)), (*GROWTH(PHYSIOLOGY), FUNGI), NUTRITION, CARBON, NITROGEN, CULTURE MEDIA, SPORES, AMINO ACIDS, CHOLESTEROL, SEX, CANADA

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE