Accession Number : AD0708382

Title :   CELL POTASSIUM BY K39 SPIN ECHO NUCLEAR MAGNETIC RESONANCE,

Corporate Author : NAVAL AIR DEVELOPMENT CENTER JOHNSVILLE PA AEROSPACE MEDICAL RESEARCH DEPT

Personal Author(s) : Cope,Freeman W. ; Damadian,Raymond

Report Date : 11 JUN 1970

Pagination or Media Count : 16

Abstract : Pulsed nuclear magnetic resonance (NMR) measurements of K39 in solutions and bacteria were made, by the use of a super-conducting magnet with a field of 50,300 gauss, which provides the necessary increase in sensitivity. Transverse relaxation time (T2) of K39 in a 1 M KC1 solution showed a mean value of approximately 0.0008 seconds. T2 of K39 in aqueous solutions increased with increasing concentrations of potassium. Fifty-nine per cent of K39 in packed wet ion exchange resin was invisible to NMR, presumably due to a marked shortening of T2 complexed by the resin. T2 of K39 in the bacterium H. halobium was shortened by at least 50 per cent, which suggests that cell K(+) is not in free solution in liquid water, but may be complexed to fixed charge sites or solvated in semicrystalline water of the cell. (Author)

Descriptors :   (*CELLS(BIOLOGY), ELECTROLYTES(PHYSIOLOGY)), (*BACTERIA, ELECTROLYTES(PHYSIOLOGY)), (*ELECTROLYTES(PHYSIOLOGY), NUCLEAR MAGNETIC RESONANCE), (*POTASSIUM, NUCLEAR MAGNETIC RESONANCE), WATER, MOLECULAR ASSOCIATION, IONS

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE