Accession Number : AD0709791

Title :   ELECTRON CYTOCHEMICAL LOCALIZATION OF CYSTINE IN PLANT CELL WALLS,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB

Personal Author(s) : Thompson,E. ; Colvin,J. Ross

Report Date : 17 DEC 1969

Pagination or Media Count : 12

Abstract : A method for specific, cytochemical staining of cystine residues was applied to thin sections of human hair keratin, two yeasts, one fungus and one green alga, for observations in the electron microscope. The method, which deposits granules of metallic silver at or close to the site of cystine residues, may be a useful means of investigating the detailed distribution of disulphide bonds in cells provided that the following criteria are rigorously applied. First, grains of metallic silver must remain after exhaustive sodium thiosulphate extraction; second, the deposition of grains of metallic silver must be inhibited by prior alkylation of the disulphide linkage. Finally, the method should be used in conjunction with other available means of analysis. (Author)

Descriptors :   (*PLANTS(BOTANY), CELL WALL), (*CELL WALL, AMINO ACIDS), (*AMINO ACIDS, *CYTOCHEMISTRY), ELECTRON MICROSCOPY, BIOLOGICAL STAINS, SILVER, CANADA

Subject Categories : Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE