Accession Number : AD0710619

Title :   CHARACTERISTICS OF A MIXED CRYOGLOBULIN.

Descriptive Note : Rept. for Dec 67-Jun 68,

Corporate Author : WILFORD HALL USAF HOSPITAL LACKLAND AFB TEX

Personal Author(s) : Gams,Richard A. ; Coltman,Charles A. , Jr

Report Date : JUN 1970

Pagination or Media Count : 22

Abstract : Some properties of a mixed cryoglobulin were investigated in this study. The cryoprotein was found to consist of a mixture of equal quantities of IgM and IgG. Optimum cryoprecipitation occurred in the pH range of 7.0 to 9.0. No complement activity could be demonstrated in the isolated cryoglobulin after incubation with normal serum and added calcium. The relative viscosity of the serum containing the mixed cryoglobulin at 37 degrees C and 15 degrees C was virtually identical to that of a serum containing an equivalent amount of a pure IgG cryoglobulin. The two components of the cryoprotein were separated by DEAE cellulose chromatography. The IgM component contained kappa light chains exclusively, whereas the IgG component contained both kappa and lambda chains. Cryoprecipitation occurred when the IgM component was incubated in the cold with sera from each of the 237 normal blood donors. (Author)

Descriptors :   (*GLOBULINS, CHEMICAL PROPERTIES), GAMMA GLOBULIN, VISCOSITY, PROTEINS, PHYSICAL PROPERTIES, IMMUNOLOGY, CHROMATOGRAPHIC ANALYSIS, CHEMICAL PRECIPITATION, MOLECULAR STRUCTURE

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE