Accession Number : AD0710862

Title :   PLANT PROTOPLASTS FOR USE IN SOMATIC CELL HYBRIDIZATION,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB

Personal Author(s) : Keller,W. A. ; Harvey,B. ; Gamborg,O. L. ; Miller,R. A. ; Eveleigh,D. E.

Report Date : 16 FEB 1970

Pagination or Media Count : 5

Abstract : Somatic cell hybridization is a potentially useful technique for the introduction of genetic variability into plant species. One proposed method involves fusion of plant protoplasts (which is a cell lacking its wall--defined here on the basis of light microscope examination) to form a hybrid cell which can be induced to form a plant. Plant protoplasts produced by the use of crude commercial lytic enzymes are not always healthy (as defined by consideration of factors such as cytoplasmic streaming, presence of cytoplasmic strands and position of the nucleus within the cell, in relation to survival time). Peroxidase, basic proteins and ribonuclease have been implicated as possible deleterious agents. The use of purified enzymes offers a more rigorously defined system for obtaining healthy protoplasts. Purified preparations of cellulase (EC 3.2.1.4), pectate lyase (EC 4.2.99.3) and endopolygalacturonase (EC 3.2.1.15) have been used to prepare protoplasts from a range of plant cell suspension cultures. Inducible streptomycete enzymes lytic towards plant cell walls have also been prepared. (Author)

Descriptors :   (*PLANTS(BOTANY), *GENETICS), (*CELLS(BIOLOGY), PLANTS(BOTANY)), ENZYMES, CYTOLOGY

Subject Categories : Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE