Accession Number : AD0712256

Title :   EXTRACELLULAR ENZYME SYSTEM UTILIZED BY THE FUNGUS CHRYSOSPORIUM LIGNORUM FOR THE BREAKDOWN OF CELLULOSE. I. STUDIES ON THE ENZYME PRODUCTION. II. SEPARATION AND CHARACTERIZATION OF THREE CELLULASE PEAKS,

Corporate Author : SVENSKA TRAFORSKNINGSINSTITUTET STOCKHOLM

Personal Author(s) : Eriksson,Karl-Erik ; Rzedowski,Wieslaw

Report Date : 30 OCT 1968

Pagination or Media Count : 13

Abstract : The production of cellulolytic and related enzymes by the fungus Chrysosporium lignorum has been studied with cellulose, xylan, and mannan as carbon sources. The enzymes studied, namely cellulase, xylanase, mannanase, beta-glucosidase and aryl-beta-glucosidase, were all found in good yield in the culture solution only when cellulose was used as carbon source. The production of cellulase and mannanase was not observed on xylan and only traces of all the enzymes studied were found on mannan as carbon source. The results of concentration, dialysis, and separation of the studied enzymes by ultrafiltration membranes are reported as well as the change in isoelectric points of the enzymes with the time of cultivation. The separation of three cellulase peaks (CMC activity) from culture solutions of the fungus Chrysosporium lignorum has also been demonstrated on a DEAE-Sephadex A-50 column. The carbohydrate content in the different peaks decreased upon dialysis to 13, 10, and 7 percent. The carbohydrate moiety consisted of mannose and traces of galactose. The hydrolysis products of cellodextrins and dewaxed cotton fibers incubated with the enzyme peaks were in all cases cellobiose and glucose in the approximate proportions 3:1. It was found that the higher the carbohydrate content of the peak, the higher the transglucosidation caused by the peak. Strong indications for the existence of a C1 enzyme in the culture solution of C. lignorum are reported. (Author)

Descriptors :   (*CELLULOSE, *DECOMPOSITION), (*ENZYMES, PURIFICATION), (*FUNGI, ENZYMES), HYDROLYSIS, CARBOHYDRATES, ION EXCHANGE, SWEDEN

Subject Categories : Biochemistry
      Wood, Paper and Related Forestry Products

Distribution Statement : APPROVED FOR PUBLIC RELEASE