Accession Number : AD0712961
Title : A BIPHASIC EXTRACTION FOR 2,8-DIOXYADENINE.
Descriptive Note : Final rept.,
Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY
Personal Author(s) : White,Sandra H. ; Loken,Michael R. ; Shields,Charles E.
Report Date : 10 APR 1970
Pagination or Media Count : 11
Abstract : A rapid quantitative method for 2,8-dioxyadenine in biological fluids has been developed using a biphasic HCl extraction from Dowex-50W, X-8, 200-400 mesh, hydrogen form. The precision and accuracy of this assay were evaluated. The least square fit of the standardization gives a slope of 0.999 plus or minus 0.0647 resulting in essentially 100% recoveries. When compared with the 2-day column chromatography technique previously available, this procedure permits numerous determinations within the hour. The result is a rapid screening assay for 2,8-dioxyadenine whenever large doses of adenine are administered. A variation of this procedure may be used as a semiquantitative test for adenine determination. (Author)
Descriptors : (*ADENINE, *ION EXCHANGE), PURINES, KETONES, URINE, BLOOD, STORAGE
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE