Accession Number : AD0712969

Title :   DISRUPTION OF YEAST MEMBRANES BY METHYLPHENIDATE.

Descriptive Note : Final rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Spoerl,Edward

Report Date : 17 JUN 1970

Pagination or Media Count : 19

Abstract : Methylphenidate blocked sorbose uptake and loss by yeast spheroplasts and, at higher concentrations, disrupted spheroplasts. At high concentrations methylphenidate also ruptured the membranes of whole yeast cells; sorbose and 280 nm-absorbing materials were lost from the cells, and methylene blue stained them. Intracellular structures were extensively affected as shown by electron micrographs, and evidently were more sensitive to methylphenidate than the external membrane. N-ethylmaleimide and Ca(++) enhanced the rupture of external membranes by methylphenidate. (Author)

Descriptors :   (*MEMBRANES(BIOLOGY), DEGRADATION), (*DRUGS, MEMBRANES(BIOLOGY)), (*YEASTS, MEMBRANES(BIOLOGY)), PERMEABILITY, CELLS(BIOLOGY), ABSORPTION(BIOLOGICAL), CELL STRUCTURE, CALCIUM, IONS, IMIDES, AMINES, HEXOSES, HETEROCYCLIC COMPOUNDS, CARBOHYDRATES

Subject Categories : Biology
      Microbiology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE