Accession Number : AD0713586

Title :   GENETIC TRANSFORMATION IN THE GENUS PASTEURELLA,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Tyeryar,Franklin J. , Jr. ; Lawton,William D.

Report Date : 1970

Pagination or Media Count : 10

Abstract : Streptomycin-sensitive cultures of Pasteurella novicida can be transformed by deoxyribonucleic acid (DNA) extracted from streptomycin-resistant cultures of P. novicida or P. tularensis. Similarly, cultures nutritionally dependent on tryptophan or adenine for growth can be transformed to nutritional independence for these requirements by DNA carrying the appropriate allele from P. novicida or P. tularensis. The formation of hybrid recombinants establishes the close genetic relatedness of P. novicida and P. tularensis, but the reduced frequency of transformation observed when P. tularensis serves as the donor suggests significant differences at the molecular level. The ability of P. novicida to undergo transformation, i.e., become competent, is not restricted to a short phase of the growth cycle, but is present uniformly throughout the logarithmic and early stationary phase of growth. (Author)

Descriptors :   (*PASTEURELLA, GENETICS), (*GENETICS, TRANSFORMATIONS), BACTERIA, CHROMOSOMES, DEOXYRIBONUCLEIC ACIDS, PASTEURELLA TULARENSIS, NUTRITION, STREPTOMYCINS, TRYPTOPHAN, ADENINE

Subject Categories : Biology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE