Accession Number : AD0713829

Title :   DETERMINATION OF ALPHA-TOCOPHEROL IN FREEZE-DRIED FOODS BY A MODIFIED COLORIMETRIC PROCEDURE.

Descriptive Note : Final rept. 1 Oct 69-30 Apr 70,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Stork,Emmett J. ; Villanueva,Ramiro P.

Report Date : SEP 1970

Pagination or Media Count : 14

Abstract : A reliably precise and reproducible determination of alpha-tocopherol was achieved by an adaptation of the classic colorimetric determination of alpha-tocopherol by Emmerie and Engel. The method was preceded by the separation of the various tocopherols either by secondary magnesium phosphate column chromatography (as described by Bro-Rasmussen and Hjarde, and Freed) or by Florisil column chromatography according to the method of Dicks-Bushnell. The basic Emmerie-Engel method, despite its disadvantages, is considered the most suitable method for use with oils, foods, and feeding stuffs. Samples of precooked, freeze-dried ground beef, chicken cubes, whole eggs, corn, beans, and peas were analyzed and alpha-tocopherol values consistent with those reported in the literature were obtained. Recovery values achieved with the addition of pure alpha-tocopherol ranged from a minimum recovery of 90.2% to a maximum recovery of 99.1%. (Author)

Descriptors :   (*VITAMIN E, *COLORIMETRIC ANALYSIS), (*DEHYDRATED FOODS, VITAMIN E), FOOD, CHEMICAL ANALYSIS, NUTRITION, CHROMATOGRAPHIC ANALYSIS

Subject Categories : Food, Food Service and Nutrition
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE