Accession Number : AD0713829
Title : DETERMINATION OF ALPHA-TOCOPHEROL IN FREEZE-DRIED FOODS BY A MODIFIED COLORIMETRIC PROCEDURE.
Descriptive Note : Final rept. 1 Oct 69-30 Apr 70,
Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX
Personal Author(s) : Stork,Emmett J. ; Villanueva,Ramiro P.
Report Date : SEP 1970
Pagination or Media Count : 14
Abstract : A reliably precise and reproducible determination of alpha-tocopherol was achieved by an adaptation of the classic colorimetric determination of alpha-tocopherol by Emmerie and Engel. The method was preceded by the separation of the various tocopherols either by secondary magnesium phosphate column chromatography (as described by Bro-Rasmussen and Hjarde, and Freed) or by Florisil column chromatography according to the method of Dicks-Bushnell. The basic Emmerie-Engel method, despite its disadvantages, is considered the most suitable method for use with oils, foods, and feeding stuffs. Samples of precooked, freeze-dried ground beef, chicken cubes, whole eggs, corn, beans, and peas were analyzed and alpha-tocopherol values consistent with those reported in the literature were obtained. Recovery values achieved with the addition of pure alpha-tocopherol ranged from a minimum recovery of 90.2% to a maximum recovery of 99.1%. (Author)
Descriptors : (*VITAMIN E, *COLORIMETRIC ANALYSIS), (*DEHYDRATED FOODS, VITAMIN E), FOOD, CHEMICAL ANALYSIS, NUTRITION, CHROMATOGRAPHIC ANALYSIS
Subject Categories : Food, Food Service and Nutrition
Distribution Statement : APPROVED FOR PUBLIC RELEASE