Accession Number : AD0714013

Title :   Studies on Stress, Toxicity and Lipid Metabolism of Erythrocytes.

Descriptive Note : Final scientific rept.,

Corporate Author : WORCESTER FOUNDATION FOR EXPERIMENTAL BIOLOGY SHREWSBURY MASS

Personal Author(s) : Ramwell,P. W.

Report Date : 1970

Pagination or Media Count : 4

Abstract : Prostaglandins at concentrations found in the circulating blood of casualties effectively increased sodium influx into human red cells and may subsequently alter erythrocyte metabolism and function. Red cells were found to readily take up prostaglandins on incubation, but no evidence was obtained to indicate that erythrocytes could biosynthesize or metabolize these compounds. The binding of prostaglandin to red cells and plasma proteins is similar in many respects to that of fatty acids. A protocol for erythrocyte incubation was designed to answer a number of questions. These were primarily (1) does the titre of various lipids in the erythrocyte change as the cell ages in storage, (2) does washing of the erythrocytes reduce the lipid titre, (3) do the erythrocytes consume, as well as biosynthesize lipids, when incubated in vitro with labelled fatty acids as substrate. The answers to all these questions now are positive for one thoroughly studied and presumably normal blood obtained from a human donor. These are preliminary results and several more normal bloods need to be studied before a reliable pattern can be described.

Descriptors :   (*ERYTHROCYTES, *STRESS(PHYSIOLOGY)), (*TOXICITY, ERYTHROCYTES), (*LIPIDS, ERYTHROCYTES), METABOLISM, CELLS(BIOLOGY), BLOOD, SHOCK(PATHOLOGY), STORAGE, BIOSYNTHESIS, VASOACTIVE AGENTS

Subject Categories : Biochemistry
      Stress Physiology
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE