Accession Number : AD0714132

Title :   A Method for Using Biotests on Albino Mice for Increasing the Sensitivity of Immunoflourescent Analysis (K Metodike Ispolvzovaniya Biioproby na Belykh Myshakh dlya Povysheniya Chuvstvitelbnosti Immunolyuminestsentnogo and Analiza),

Corporate Author : ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER WASHINGTON D C

Personal Author(s) : Prusakova,Z. M.

Report Date : 07 JUL 1970

Pagination or Media Count : 6

Abstract : A study was undertaken for investigating the possibility of using tests on laboratory animals as a biological method for accumulating microorganisms for their subsequent detection by the fluorescent antibodies method. The objective was to compare and evaluate the effectiveness of using hydrocortisone, adrenaline and egg yolk for increasing the sensitivity of immunofluorescent analysis. The test microbes used were the positive agents of Siberian plague and tularemia. It was found that in the immunofluorescent analysis of unknown material the substance insuring a rapid accumulation of different microorganisms in the organs of albino mice was hydrocortisone; egg yolk can be recommended only for detecting the positive agent of Siberian plague; the use of adrenaline for increasing the sensitivity of albino mice to microbes is undesirable. (Author)

Descriptors :   (*FLUORESCENT ANTIBODY TECHNIQUES, SENSITIVITY), (*BIOASSAY, MICROORGANISMS), ANTIGENS + ANTIBODIES, DETECTION, PASTEURELLA TULARENSIS, BACILLUS ANTHRACIS, CORTICOSTEROID AGENTS, EPINEPHRINE, IMMUNOLOGY, MICE, EGGS, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE