Accession Number : AD0714344

Title :   Transcription Properties of Stepwise Acid-Extracted Chicken Erythrocyte Chromatin,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB

Personal Author(s) : Seligy,V. L. ; Neelin,J. M.

Report Date : 09 MAR 1970

Pagination or Media Count : 12

Abstract : The template capacity of chromatin prepared from mature erythrocyte nuclei by extraction with saline solutions of progressively lower pH values was determined. Histones contained in both the nuclear extracts and the resultant chromatins were examined by starch-gel electrophoresis. No change in template capacity was observed after the removal of lysinerich histone. Approx. 40-50% of the template capacity of fully dehistonized chromatin was obtained by the removal of the serine-rich histone, while displacement of the arginine-rich and moderately lysine-rich histones together accounted for the remainder of the increase in transcription. The influence of acid depurination on the transcription properties of this system was examined. Residual non-histone proteins were found to inhibit DNA transcription by an additional 21-25% of the total capacity of deproteinized DNA. (Author)

Descriptors :   (*ERYTHROCYTES, HISTONES), (*HISTONES, *GENETICS), CHICKENS, TEMPLATES, DEOXYRIBONUCLEIC ACIDS, ELECTROPHORESIS, THIN LAYER CHROMATOGRAPHY, SOLVENT EXTRACTION

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE