Accession Number : AD0715177

Title :   A Comparison of the Soluble Proteins in Various Species of Algae by Disc Electrophoresis in Polyacrylamide Gels,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA HALIFAX (NOVA SCOTIA) ATLANTIC REGIONAL LAB

Personal Author(s) : Young,E. Gordon

Report Date : 20 DEC 1969

Pagination or Media Count : 9

Abstract : Aqueous saline extracts of fresh specimens of twelve species of marine algae, representative of the Chlorophyceae, Rhodophyceae and Phaeophyceae, have been analyzed by disc electrophoresis in polyacrylamide gels. The pattern of proteins in the green algae was the most complex with about 12 bands, that in the reds less complex with about 9 bands and least in the browns with about 5 bands. Fractionation of the extracts with (NH4)2SO4 indicated the probable presence of several globulins and chromoproteins, and two albumins, but such fractions were grossly heterogeneous. Fractionation of the extract from Rhodymenia palmata indicated the presence of allophycocyanin, R-phycocyanin and R-phycoerythrin mainly from spectrometric data. Trials with gradient gels and isoelectric focusing were less informative than by the conventional method of electrophoresis in 7.5% gels. Comparison of six stains for algal proteins indicated Coomassie Brilliant Blue and Woolfast Blue as preferable. (Author)

Descriptors :   (*ALGAE, *PROTEINS), ELECTROPHORESIS, ACRYLIC RESINS, CHEMICAL ANALYSIS, CANADA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE