Accession Number : AD0715179

Title :   The Nature of Some Solvent-Derived Artifact Spots Observed During the Chromatography of Adrenaline,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA HALIFAX (NOVA SCOTIA) ATLANTIC REGIONAL LAB

Personal Author(s) : Forrest,Janet E. ; Heacock,R. A. ; Roberts,D. J. ; Forrest,T. P.

Report Date : 01 JUN 1970

Pagination or Media Count : 9

Abstract : Several spots are often detected on chromatograms obtained from solutions of a single pure beta-(3,4-dihydroxyphenyl)ethanolamine derivative, such as adrenaline, particularly when the base is dissolved in strong acids prior to chromatography. The formation of some of these multiple spots can be explained by either the formation of complexes or salts, involving the catecholamine and the acids employed, or by the chemical modification of the catecholamine prior to chromatography. It has now been shown that substances responsible for certain artifact spots are formed by the interaction of one of the components of the chromatographic solvent system used with a reactive intermediate derived from the beta-phenylethanolamine derivative being chromatographed. For example, adrenaline n-butyl ether is formed during the chromatography of adrenaline in n-butanol-hydrochloric acid solvent mixtures and beta-(p-hydroxyphenyl)-beta-(3',4'-dihydroxyphenyl)ethylmethylamine is one of the artifact spots observed when adrenaline is chromatographed in phenol-hydrochloric acid systems. (Author)

Descriptors :   (*EPINEPHRINE, *THIN LAYER CHROMATOGRAPHY), AMINES, IMPURITIES, CHEMICAL REACTIONS, CANADA

Subject Categories : Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE