Accession Number : AD0715993

Title :   A Study of the Biological Properties of Trypanosoma rhodesiense and Trypanosoma gambiense.

Descriptive Note : Final rept. no. 3, 1 Jun 69-31 Oct 70,

Corporate Author : TULANE UNIV NEW ORLEANS LA DEPT OF BIOLOGY

Personal Author(s) : Seed,John Richard

Report Date : JUN 1969

Pagination or Media Count : 26

Abstract : Three enzymes, hexokinase, aldolase and phosphohexoisomerase, were partially purified by a combination of (NH4)2SO4 fractionation, and DEAE-Sephadex column chromatography. These enzymes were biologically and physically characterized. Since these enzymes are critical trypanosome enzymes, neutralizing rabbit antisera prepared against them have been tested for their ability to passively protect mice. No protection was observed. In addition by similar techniques a protective antigen was partially purified from crude extracts of Trypanosoma gambiense and Trypanosoma equiperdum the fractions in which the protective antigen(s) was found were identical for both species. The immunization of mice with this antigen lead to significant increases in survival times, and up to 100% of the animals were completely protected. The addition of complete Freund adjuvant to this antigen failed to increase the survival times, or percent survival. (Author)

Descriptors :   (*TRYPANOSOMA, *IMMUNOLOGY), (*ENZYMES, TRYPANOSOMA), ANTIGENS + ANTIBODIES, IMMUNE SERUMS, IMMUNITY, PREPARATION, RESPONSE(BIOLOGY), MICE, RABBITS, PARASITES, PARASITIC DISEASES, VACCINES, AFRICA, PHOSPHORUS TRANSFERASES, ALDEHYDE LYASES, ISOMERASES

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE