Accession Number : AD0716602

Title :   A Study on the Complex of Soluble Proteins in the Cells of Clostridium Perfringens by Electrophoresis in Polyacrylanide Gel (O Komplekse Rastvorimykh Belkov, Soderzhashchikhsya v Kletke Clostridium Pereringens),

Corporate Author : ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER WASHINGTON D C

Personal Author(s) : Nikolaeva,S. A. ; Safonov,V. I.

Report Date : 14 OCT 1970

Pagination or Media Count : 8

Abstract : The method of electrophoresis in polyacrylamide gel is suitable for separating soluble acid cell proteins. The proteins are separated into a great many sharply demarcated zones and the resultant 'protein spectra' are analyzed. As a rule, the strains of C. Perfringens isolated from various sources differ considerably in their protein spectra. Correlation is evident between the electrophoretic data of various strains of C. Perfringens and certain of their biological properties, primarily toxigenicity. In many instances, the most toxic strains have a specific, clearly marked zone. A change in the protein spectra of the investigated strains of C. perfringens is observed when the composition of the nutrient medium is altered (enriched with casein hydrolystate). The synthesis of certain proteins increases markedly and new proteins appear. (Author)

Descriptors :   (*CLOSTRIDIUM PERFRINGENS, PROTEINS), (*PROTEINS, ELECTROPHORESIS), IDENTIFICATION, BACTERIA, CHEMICAL ANALYSIS, CLASSIFICATION, USSR, TOXICITY, ACRYLIC RESINS

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE