Accession Number : AD0716657

Title :   A Study of the Structural Integrity of Spin-Labelled Proteins in Some Fractions of Human Erythrocyte Ghosts,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB

Personal Author(s) : Schneider,Henry ; Smith,Ian C. P.

Report Date : 06 APR 1970

Pagination or Media Count : 9

Abstract : The spin label method has been applied to the problem of isolating membrance proteins without altering their structure. Spin-labelled erythrocyte ghosts were dissolved using eight different procedures, and the electron spin resonance spectra of the resulting fractions studied prior to and after removal of solubilizer. Marked differences were observed in the spectral perturbations caused by the various dissolution procedures. However, in a number of instances fractions were obtained after removal of solubilizer whose spectra resembled that of the labelled ghosts, suggesting that the structural integrity of the labelling site had been largely regained. These results are taken to indicate the fractions which are appropriate departure points for subsequent more detailed isolution studies where retention of structural integrity is of interest. The data also suggested mechanisms by which the various solubilizers operate, and that the structures of some proteins are not highly dependent on the lipids which can be extracted by n-butanol. (Author)

Descriptors :   (*PROTEINS, *ELECTRON PARAMAGNETIC RESONANCE), (*MEMBRANES(BIOLOGY), PROTEINS), SOLUTIONS(MIXTURES), CHEMICAL ANALYSIS, SOLVENT ACTION, CANADA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE