Accession Number : AD0716962

Title :   The Use of a Carbocyanine Dye to Detect Trace Amounts of Protein in Biological Media.

Descriptive Note : Final rept.,

Corporate Author : NAVAL RESEARCH LAB WASHINGTON D C

Personal Author(s) : Michajluk,Beverly Jae ; Loeb,George I.

Report Date : 21 DEC 1970

Pagination or Media Count : 16

Abstract : To test its applicability as a detector of biopolymers, a carbocyanine dye was allowed to react with proteins, polypeptides, polysaccharides, lipids, and supernatants from marine cultures, and changes in the visible spectra were observed. beta-Lactoglobulin and gelatin have thus far proved satisfactory as standards; the smallest detectable concentration was 2 ppm. Since different spectral features become prominent when the type of polymer or the state of the polymer is varied, it may be possible to classify marine polymers in very dilute solution by spectral means. Evidence has been found for considering a peak at 605 nm as characteristic of a denatured protein. (Author)

Descriptors :   (*BIOLOGICAL STAINS, COLORIMETRIC ANALYSIS), (*PROTEINS, *COLORIMETRIC ANALYSIS), (*DYES, BIOLOGICAL STAINS), (*CARBOHYDRATES, COLORIMETRIC ANALYSIS), MARINE BIOLOGY, POLYMERS, ULTRAVIOLET SPECTRA, VISIBLE SPECTRA, PEPTIDES, LIPIDS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE