Accession Number : AD0718159

Title :   Studies on Proteinases from Some Blood-Sucking Insects,

Corporate Author : ALBERTA UNIV EDMONTON DEPT OF ENTOMOLOGY

Personal Author(s) : Gooding,R. H.

Report Date : 1969

Pagination or Media Count : 8

Abstract : By use of synthetic substrates, trypsin and chymotrypsin have been demonstrated in the midguts of blood-fed Aedes aegypti, Culex fatigans, Melophagus ovinus, and Pediculus humanus, but not in those of Cimex lectularius or Rhodnius prolixus. The trypsin and chymotrypsin have been partially separated by Sephadex Gel filtration. Both enzymes are inhibited by phenylmethyl sulfonyl-fluoride and soybean trypsin inhibitor. The trypsins are inhibited by N-a-p-tosyl-lysine chloromethyl ketone but not by L-1-tosylamide-2-phenyl-ethylchloromethyl ketone. The reverse is true for the chymotrypsins. Cimex and Rhodnius appear to have a high molecular weight proteinase with optimal activity at pH 5 in their midguts. (Author)

Descriptors :   (*PEPTIDE HYDROLASES, *INSECTS), (*ENZYMES, INSECTS), ENTOMOLOGY, DIGESTIVE SYSTEM, PH FACTOR, TRYPSIN, CHYMOTRYPSIN, AEDES, CULEX, CULICIDAE, LICE, HEMIPTERA, DIPTERA

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE