Accession Number : AD0719287

Title :   Interaction Between Arbovirus and Myxovirus.

Descriptive Note : Annual rept. no. 4, 3 Nov 69-2 Nov 70,

Corporate Author : KURUME UNIV (JAPAN) DEPT OF MICROBIOLOGY

Personal Author(s) : Nakamura,Masahiro

Report Date : JAN 1971

Pagination or Media Count : 30

Abstract : The investigations concerning the 'markers' of viruses to be used for recombination or mutation experiments followed the studies carried out during the first half of this year. For the investigations, Arbovirus group A Sindbis virus was mainly used. First, biological differences between Sindbis virus, JEV, and NWS strain of influenze virus were studied. From the results, Sindbis virus was more resistant to acid (pH 3) and heat than that of NWS virus. Second, a Sindbis virus mutant having a character capable of production of a large and clear plaque was obtained by passage of the virus 50 times through chorioallantoic membrane (CAM) tissue culture. Third, it was indicated that CAM passaged 20 times Sindbis virus had some different properties when adsorbed to cell monolayers, comparing to Sindbis chick embryo brain (CEB) passaged virus, i.e. CAM virus needed Mg and Ca more than CEB virus for adsorption to the cells. (Author)

Descriptors :   (*ARBOVIRUSES, GENETICS), (*MYXOVIRUSES, GENETICS), (*GENETICS, *VIRUSES), MUTATIONS, INHIBITION, INFLUENZA VIRUS

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE