Accession Number : AD0719702

Title :   Hemoglobin Function in Stored Blood. VI. The Effect of Phosphate on Red Cell ATP and 2,3-DPG.

Descriptive Note : Interim rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Dawson Jr.,Ben ; Kocholaty,Walter F.

Report Date : 22 DEC 1970

Pagination or Media Count : 13

Abstract : The objective of the study was to determine if a higher concentration of inorganic phosphate than 2mM (CPD: citrate-phosphate-dextrose) would benefit red cell concentrations of ATP and 2,3-diphosphoglycerate during blood storage. Blood preservatives with phosphate concentrations of 2 mM (CPD), 5 mM, 10mM, 15mM, and 20 mM were used to study red cell preservation under blood bank storage conditions at 4 C. Procedural and analytic methods previously employed in these laboratories were employed. Concentrations of phosphate higher than 2 mM (CPD) were found to be of no benefit for maintenance of ATP and 2,3-DPG during 5 weeks of storage. (Author)

Descriptors :   (*BLOOD, STORAGE), (*HEMOGLOBIN, PHOSPHATES), ADENOSINE PHOSPHATES, GLYCEROLS, GLYCOLYSIS, ERYTHROCYTES

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE