Accession Number : AD0719992

Title :   Stimulation of Epinephrine of Adenyl Cyclase Activity, Cyclic AMP Foundation, DNA Synthesis and Cell Proliferation in Populations of Rat Thymic Lymphocytes,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGY

Personal Author(s) : MacManus,J. P. ; Whitfield,J. F. ; Youdale,T.

Report Date : 26 AUG 1970

Pagination or Media Count : 14

Abstract : During the first ten minutes after the beginning of a continuous exposure of rat thymocyte populations (maintained in vitro) to epinephrine, there is an increase in the cellular concentration of cyclic AMP. The hormone also increases the activity of a crude preparation of the thymocyte's cyclic AMP-forming enzyme, adenyl cyclase. Between 30 and 45 minutes after the beginning of exposure to epinephrine, an additional part of the cell population begins to synthesize deoxyribonucleic acid (DNA). These changes are finally followed two to four hours later by an increase of the flow of cells into mitosis. Since cyclic AMP itself is known to stimulate both the initiation of DNA synthesis and thymocyte proliferation, and since the mitogenic action of epinephrine is shown to be potentiated by caffeine and inhibited by imidazole, it is concluded that the mitogenic action of this hormone is mediated by the cyclic nucleotide. (Author)

Descriptors :   (*EPINEPHRINE, *CELL DIVISION), (*LYMPHOCYTES, EPINEPHRINE), CALCIUM, HORMONES, THYMUS, CONCENTRATION(CHEMISTRY), ADENOSINE PHOSPHATES, CANADA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE