Accession Number : AD0719993

Title :   The Metabolism of Exogenous Cyclic AMP at Low Concentrations by Thymic Lymphocytes,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGY

Personal Author(s) : MacManus,J. P. ; Whitfield,J. P. ; Braceland,B.

Report Date : 22 DEC 1970

Pagination or Media Count : 8

Abstract : When isolated thymic lymphocytes were incubated with known mitotically stimulating concentrations of a mixture of cyclic AMP labeled with 32phosphorus and tritium, 0.5% of the phosphorus label and 3 to 6% of the tritium label entered the cells. None of the radioactivity from either isotope appearing in the cells was present as cyclic AMP. This difference between the entry of the two iostopes was explained by the observation that the cyclic AMP in the medium surrounding the cells was metabolized, and it was breakdown products of the cyclic nucleotide which entered the cells. It is concluded that cyclic AMP does not enter cells as such, and may exert its action on mitosis at the cell surface. (Author)

Descriptors :   (*THYMUS, LYMPHOCYTES), (*LYMPHOCYTES, ADENOSINE PHOSPHATES), (*CELLS(BIOLOGY), *ADENOSINE PHOSPHATES), (*MITOSIS, CELLS(BIOLOGY)), LABELED SUBSTANCES, DEOXYRIBONUCLEIC ACIDS, IN VITRO ANALYSIS, CANADA

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE