Accession Number : AD0726335

Title :   Hemoglobin Function in Stored Blood: VIII. Further Effects of Phosphate on Red Cell ATP and 2,3-DPG.

Descriptive Note : Progress rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Dawson,Ben , Jr. ; Kocholaty,Walter F.

Report Date : 29 MAR 1971

Pagination or Media Count : 12

Abstract : Inorganic phosphate which is known to stimulate red cell glycolysis is present in one of the preservatives for storing whole blood, citrate-phosphate-dextrose (CPD), but not the other, acid-citrate-dextrose (ACD). Both of these preservatives for liquid storage were developed before 2,3-diphosphoglycerate (2,3-DPG) was found to be necessary for normal hemoglobin function. In a recent study we have shown that very high concentrations of phosphate (10, 15, and 20 mM) were deleterious for maintaining 2,3-DPG. In the present study a lower range of phosphate concentrations (2, 4, 6, and 9 mM) was studied for maintenance of 2,3-DPG and ATP during storage under blood banking conditions. The lowest concentration, 2 mM, which corresponds to CPD was found to be the best concentration for maintaining 2,3-DPG and thus hemoglobin function. Four mM phosphate were not quite as good but better than no phosphate. Six and 8 mM phosphate were considerably worse. (Author)

Descriptors :   (*BLOOD, STORAGE), (*HEMOGLOBIN, *PHOSPHATES), (*ERYTHROCYTES, PHOSPHATES), ADENOSINE PHOSPHATES, GLYCOLYSIS, BIOCHEMISTRY, HEMATOLOGY, BLOOD TRANSFUSION

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE