Accession Number : AD0728285

Title :   The Non-Uniform Distribution of Proteins in Plant Cell Walls,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB

Personal Author(s) : Colvin,J. Ross ; Leppard,Gary G.

Report Date : 1971

Pagination or Media Count : 20

Abstract : The distribution of disulphide groups in the cell walls of suspension cultured cells of Ipomoea, Phaseolus and Daucus was determined by the electron microscope using a modification of the Gomori silver-hexamine reagent. If the distribution of the disulphide groups is roughly equated with the distribution of disulphide containing proteins, the results show considerable variation in the distribution of protein in the cell wall, both within one species and between species. Cell walls of Ipomoea have a minimum of concentration of protein in the center of the wall; Phaseolus cell walls show little variation and the cell walls of Daucus show increasing concentration from the inside to the outside. Fine lamellation of the protein sometimes occurs even in the thinnest walls and occasionally there are gaps in the local distribution of protein. The cause of these variations is unknown. (Author)

Descriptors :   (*CELL WALL, *PLANTS(BOTANY)), (*PROTEINS, CELL WALL), (*CYTOCHEMISTRY, PLANTS(BOTANY)), SULFIDES, ELECTRON MICROSCOPY, CYTOLOGY, CANADA

Subject Categories : Biochemistry
      Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE