Accession Number : AD0729305

Title :   Hemoglobin Function in Stored Blood. IX. A Modified Preservative with Optimal pH to Maintain Red Cell 2,3-DPG (Function) and ATP (Viability).

Descriptive Note : Progress rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Dawson,Ben , Jr. ; Loken,Michael R. ; Crater,Davis H. ; Newlon,David ; Miller,Gordon F.

Report Date : 20 MAY 1971

Pagination or Media Count : 18

Abstract : After demonstrating that hemoglobin function (p50 and 2,3-DPG) was better maintained with blood stored in CPD as compared to ACD, the senior author showed that the higher pH of CPD was the reason for its better maintenance of hemoglobin function. In the present study a wider range of preservative pHs is studied to determine the optimal pH for maintaining 2,3-DPG or hemoglobin function and ATP or red cell viability, during liquid storage under blood banking conditions. An automated analytical system for determining concentrations of 2,3-DPG and ATP was used in the study of ten units from normal volunteers. Each unit was split during donation into five parts containing citrate-dextrose solutions of pH 5.0, 5.5, 6.0, 6.5, and 7.0. Significant differences at the 95 percent level were based on the paired 't' test. In addition, osmotic fragility and methylene blue uptake were determined to assess their possible usefulness as indicators of red cell viability or ATP. 2,3-DPG fell from day 0 to day 3 in the pH 5.0 preservative, and from day 3 to day 7 in pH 5.0 and 5.5 preservatives. In conclusion, from the 2,3-DPG and ATP data a preservative with a pH higher than 5.5 would seem to be optimal for maintaining hemoglobin function and red cell viability. In addition, methylene blue uptake seems to correlate fairly well with maintenance of ATP in the two lowest pH preservatives. (Author)

Descriptors :   (*BLOOD, STORAGE), (*HEMOGLOBIN, PHYSIOLOGY), (*PH, BLOOD), PRESERVATION, ERYTHROCYTES, CITRATES, GLUCOSE, PHOSPHATES, ADENOSINE PHOSPHATES

Subject Categories : Medical Facilities, Equipment and Supplies

Distribution Statement : APPROVED FOR PUBLIC RELEASE