Accession Number : AD0732654
Title : Separation of Gibberellin Al and Dihydrogibberellin Al by Argentation Partition Chromatography on a Sephadex Column,
Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA HALIFAX (NOVA SCOTIA) ATLANTIC REGIONAL LAB
Personal Author(s) : Vining,L. C.
Report Date : 10 MAY 1971
Pagination or Media Count : 3
Abstract : In samples of (3,4-(3)H) gibberellin A(1) prepared by partial reduction of gibberellin A(3) with tritium the fully saturated dihydrogibberellin A(1) isomers are frequent contaminants. Silver nitrate is widely used as a complexing agent in adsorption chromatography to separate substances differing only in the number and geometry of their double bonds, but can also alter the partition coefficients of such compounds. Because many gibberellins are conveniently separated by partition chromatography on Sephadex columns the author examined the effect of silver ion complexing on the distribution of gibberellin A(1) and its dihydro derivatives. Whereas a mixture of these compounds was not resolved in the unsupplemented solvent systems tested, addition of silver nitrate altered the partition coefficient and permitted a separation of gibberellin A(1) from the fully saturated components.
Descriptors : (*GROWTH SUBSTANCES, *THIN LAYER CHROMATOGRAPHY), SILVER COMPOUNDS, NITRATES, ADDITIVES, PURIFICATION, CANADA
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE