Accession Number : AD0732759
Title : Detection and Semi-Quantitative Estimation of Free Morphine in Human Urine by Spectrofluorometry.
Descriptive Note : Medical research interim rept.,
Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD
Personal Author(s) : Rodkey,Frederick Lee ; Collison,Harold A. ; Petrin,Matthew J. ; O'Neal,John D.
Report Date : 07 SEP 1971
Pagination or Media Count : 15
Abstract : A simplified procedure for detection of free morphine in human urine by use of spectrofluorometry is described. The procedure involves (a) extraction of morphine from urine at pH 8.5-9 into an organic phase, (b) treatment of the organic phase with sodium hydroxide to remove morphine and other phenolic compounds from the organic phase into the aqueous hydroxide. This simple extract is added to a boric acid-borate mixture for spectrophotofluorometric analysis directly and after addition of potassium ferricyanide. The ferricyanide of pH about 9 converts morphine to pseudo-morphine with a marked increase in fluorescence. The procedure detects only free unconjugated morphine. It is sufficiently sensitive to detect one microgram per ml of urine or less and can be performed in a semi-quantitative manner. False positive reactions are minimized by the solubility requirements and by utilizing the effects of a specific chemical reaction on the fluorescence. (Author)
Descriptors : (*MORPHINE, URINE), (*URINE, CHEMICAL ANALYSIS), (*SPECTROSCOPY, URINE), FLUOROMETERS, DETECTION, QUANTITATIVE ANALYSIS, NARCOTICS
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE