Accession Number : AD0734827

Title :   A Fluorometric Assay of Methyl Ketones.

Descriptive Note : Technical rept. Jan-Mar 71,

Corporate Author : EDGEWOOD ARSENAL MD

Personal Author(s) : Kramer,David N. ; Tolentino,Lucio U. ; Hackley,Ethel B.

Report Date : DEC 1971

Pagination or Media Count : 18

Abstract : A quantitative kinetic fluorescence method is reported for alpha-methyl ketones, e.g., acetone and acetophenone. The reaction involves the condensation of o-nitrobenzaldehyde with the alpha-methyl ketones at pH 12.5 to form the fluorescent indoxyl. The rate of fluorescence formation was shown to be linear with respect to methyl ketone at low concentrations. The rate limiting step in the reaction is the one involving the alpha-methyl ketone anion attack on the o-nitrobenzaldehyde. Photolysis of the adduct of the methyl ketone and o-nitrobenzaldehyde likewise yielded indoxyl. Isopropanol by prior oxidation to the ketone was detected also. (Author)

Descriptors :   (*KETONES, CHEMICAL ANALYSIS), FLUORESCENCE, FLUOROMETERS, REACTION KINETICS, ACETONES, ACETOPHENONES

Subject Categories : Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE