Accession Number : AD0736479

Title :   Cytological Study of Peritoneal Exudate of Mice in the Course of Interferon Production in Vitro.

Corporate Author : ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA

Report Date : 27 OCT 1971

Pagination or Media Count : 16

Abstract : Ribonucleoprotein complexes, possessing infectious properties and capable of forming characteristic plaques under agar, are formed in the mitochondrial fraction, isolated from cells infected with Venezuelan equine encephalomyelitis virus (VEEV) in the course of incubation in appropriate media. In the linear saccharose gradient, the sedimentation constants of these complexes varied from 80 S to 160 S, while their buoyant density in the CxCl gradient was 1.3 to 1.42 grams per cc. Virus-like particles ('pseudoviruses') with the same characteristics were also found when infectious RNA VEEV was added to a homogenate obtained from non-infected cells. They were partially resistant to ribonuclease and were not neutralized by virus-specific sera. It is suggested that on the basis of the formation of virus-like particles in systems in vivo and in vitro there is complexing between the RNA viruses and the cell proteins. Formation of informosome-type structures is possible. (Author)

Descriptors :   (*NUCLEOPROTEINS, VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS), PROTEINS, MITOCHONDRIA, CELLS(BIOLOGY), RIBONUCLEIC ACIDS, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE