Accession Number : AD0739626

Title :   A Spin Label Study of the Organization and Fluidity of Hydrated Phospholipid Multibilayers - a Model Membrane System,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) BIOCHEMISTRY LAB

Personal Author(s) : Smith,Ian C. P.

Report Date : 1971

Pagination or Media Count : 12

Abstract : The essential theory of the spin label method is presented and applied to the case of a rigid spin label localized in an oriented phospholipid smectic mesophase (hydrated phospholipid multibilayers). The fatty acid chains of the phospholipids in a model membrane of egg lecithin are shown to make an average angle of 24 deg with respect to a perpendicular to the bilayer plane. Addition of cholesterol stiffens the bilayers and decreases this angle. The various requirements of a sterol for this condensing effect are explored. Ions, proteins, anesthetics, antibiotics, and decouplers of oxidative phosphorylation are shown to have profound effects on the structure of lipid bilayers. (Author)

Descriptors :   (*MEMBRANES(BIOLOGY), DEFORMATION), (*ELECTRON PARAMAGNETIC RESONANCE, *PHOSPHOLIPIDS), MOLECULAR STRUCTURE, CHOLESTEROL, HYPERFINE STRUCTURE, MIXTURES, THEORY, CANADA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE