Accession Number : AD0745800

Title :   Characterization of L-leucine Induced Germination of Trichophyton mentagrophytes microspores.

Descriptive Note : Technical rept.,

Corporate Author : STRITCH SCHOOL OF MEDICINE MAYWOOD ILL DEPT OF MICROBIOLOGY

Personal Author(s) : Hashimoto,T. ; Wu,C. D. R. ; Blumenthal,H. J.

Report Date : 31 MAR 1970

Pagination or Media Count : 45

Abstract : L-Leucine and several amino acids proved to be effective germination inducers of microspores of Trichophyton mentagrophytes. During germination phase darkening and swelling occurred concomitantly with the alterations of several spore properties inherent to dormancy. Such changes included the loss of resistance to heat and stain, the reduction of dry weight and specific gravity, and the development of active glucose utilization. The germination induced by L-leucine was significantly stimulated by a pre-treatment of the spores with sublethal doses of heat. D-leucine proved to be almost equally effective germinant as its L-form. Other factors that affected the L-leucine induced germination of the microspores included the concentration of leucine, the inoculum size of the spores, temperature and pH. The anaerobic condition and the presence or absence of carbon dioxide had no significant effects on the germination. (Author)

Descriptors :   (*FUNGI, SPORES), (*SPORES, GERMINATION), (*AMINO ACIDS, FUNGI), STIMULATION(PHYSIOLOGY), PHYSIOLOGY, TEMPERATURE, PH FACTOR, CYTOLOGY

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE