Accession Number : AD0746489

Title :   Control of Flies and Cockroaches by Insect Hormones.

Descriptive Note : Annual rept. no. 1, 1 Aug 71-31 Jul 72,

Corporate Author : NORTHWESTERN UNIV EVANSTON ILL DEPT OF BIOLOGICAL SCIENCES

Personal Author(s) : Gilbert,Lawrence I.

Report Date : 10 AUG 1972

Pagination or Media Count : 13

Abstract : The research described deals with two subjects; hormone receptors in insects and the tanning of the insect cuticle. If either process can be interfered with, one would have an effective means of insect control. Receptor studies using sucrose density gradient ultracentrifugation demonstrated the existence of two protein receptors in the crustacean hepatopancaeas that bind label from 3H-ecdysone (molting hormone). Microchemical analysis demonsttated that the label was now due to a previously undescribed ecdysone metabolite. Experiments with the lipoidal juvenile hormone showed that a specific hemolymph (blood) lipoprotein binds 14C-juvenile hormone. Using column chromatography, electrophoresis, and immunological procedures, the author has shown that 14C-dopamine (a central compound in tanning of the cuticle) is metabolized by two different routes in the tobacco hornworm. The immunochemical and radiotracer studies demonstrate for the first time that these hemolymph carrier proteins do indeed traverse the epidermal cell to make their way unaltered into the cuticle undergoing tanning. (Author)

Descriptors :   (*INSECT CONTROL, *HORMONES), (*BLATTIDAE, INSECT CONTROL), BLOOD PROTEINS, CHEMICAL BONDS, LABELED SUBSTANCES, MUSCIDAE, CHROMATOGRAPHIC ANALYSIS, METABOLISM, BIOCHEMISTRY

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE