Accession Number : AD0746840

Title :   Changes in Red Cells Following Rapid Freezing with Extracellular Cryoprotective Agents,

Corporate Author : AMERICAN NATIONAL RED CROSS BETHESDA MD BLOOD RESEARCH LAB

Personal Author(s) : Meryman,H. T. ; Hornblower,M.

Report Date : 1971

Pagination or Media Count : 23

Abstract : The recovery of human red cells following slow freezing requires the presence of a high concentration of a penetrating cryoprotective agent. However, if the freezing rate is relatively rapid a variety of nonpenetrating compounds will provide good cryoprotection at low concentrations. Freezing rates which produce optimum recovery are all rapid and require liquid nitrogen temperature for storage. The mechanism by which freezing injures living cells has been extensively investigated. A discussion is made of a series of experiments undertaken to determine whether an exchange of extracellular and intracellular electrolytes might be a necessary component of the recovery of red cells following rapid freezing with extracellular cryoprotective agents.

Descriptors :   (*ERYTHROCYTES, *FREEZING), BLOOD, PROTECTION, STABILIZATION, DEHYDRATION, PRESERVATION, CHEMICAL COMPOUNDS

Subject Categories : Medicine and Medical Research
      Medical Facilities, Equipment and Supplies

Distribution Statement : APPROVED FOR PUBLIC RELEASE