Accession Number : AD0746898

Title :   Preservation of Mammalian Tissues and Organs by Freezing.

Descriptive Note : Final rept.,

Corporate Author : AMERICAN NATIONAL RED CROSS BETHESDA MD BLOOD RESEARCH LAB

Personal Author(s) : Meryman,Harold T.

Report Date : 30 JUN 1972

Pagination or Media Count : 13

Abstract : Further support has been obtained for the minimum volume hypothesis of freezing to mammalian cells. When freezing is slow with extracellular ice the injury results primarily from the concentration of extracellular solutes and is apparently related to their osmotic effects. Studies with potassium-depleted human red cells show that injury is unrelated to the absolute osmolality of the suspending solutions or the concentration of intracellular electrolyte. Injury beings to occur with the loss of 65% of the normal cell water content. Spinach chloroplast grana do not reach critical volume and remain uninjured at any concentration or temperature. It is proposed that cells mechanically resist volume change and that in hypertonic suspension they are not in equilibrium with their environment but have an osmotic pressure gradient across the membrane. (Author)

Descriptors :   (*CELLS(BIOLOGY), FREEZING), (*TISSUES(BIOLOGY), PRESERVATION), DAMAGE, BLOOD CELLS, THEORY, OSMOTIC PRESSURE, VOLUME, MEMBRANES(BIOLOGY), STABILIZATION, CYTOLOGY

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE